Ultrafast and absolute quantification of SARS-CoV-2 on food using hydrogel RT-LAMP without pre-lysisArticle Published on 2023-01-152022-11-16 Journal: Journal of hazardous materials [Category] SARS, 치료기술, [키워드] Absolute quantification acute respiratory syndrome amplification cold chain Cold chain food contact infection coronavirus faster Food industry hydrogel Hydrogel RT-LAMP isothermal amplification microbe offer POCT point-of-care quantification reproducibility reverse transcription RNA RNA extraction RT-LAMP SARS-CoV-2 sensitivity specificity stability Surveillance Ultrafast detection. virus [DOI] 10.1016/j.jhazmat.2022.130050 PMC 바로가기
Reverse transcriptase-free detection of viral RNA using Hemo Klentaq DNA polymeraseArticle Published on 2022-12-152022-11-15 Journal: Analytical biochemistry [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] affected amplification Analysis can be used caused comparable COVID-19 Degraded RNA demonstrated determine DNA polymerase Efficiency freeze-thaw cycles limitations Nasopharyngeal swab Nearby Nearby primers overcome pandemic patients PCR polymerase chain polymerase chain reaction Primers provide provided reliability reverse transcriptase Reverse transcriptase activity reverse transcription RNA RT-PCR SARS-CoV-2 SARS-CoV-2 detection target transcriptase Viral RNA Viral RNA. [DOI] 10.1016/j.ab.2022.114960 PMC 바로가기
Highly sensitive and rapid detection of SARS-CoV-2 via a portable CRISPR-Cas13a-based lateral flow assayArticle Published on 2022-12-012022-11-15 Journal: Journal of medical virology [Category] COVID19(2023년), SARS, 치료기술, [키워드] acute respiratory syndrome addition amplification approach assays clinical sample comparable component Concentration coronavirus disease Coronavirus-2 COVID-19 CRISPR Diagnostic method Effectiveness ENhance examined identify immunochromatographic IMPROVE individual isothermal lateral flow lateral flow assay lateral flow strip limit of detection molecular polymerase chain primer probe Protein provide Quality control reliability Result reverse transcription RNA RT-PCR SARS-CoV-2 screened sensitive detection. sensitivity significantly Spread tested virus detection [DOI] 10.1002/jmv.28096 PMC 바로가기
Current trends in COVID-19 diagnosis and its new variants in physiological fluids: Surface antigens, antibodies, nucleic acids, and RNA sequencingReview article Published on 2022-12-012022-10-05 Journal: TrAC Trends in Analytical Chemistry [Category] 바이오마커, 변종, 진단, 치료기술, [키워드] addressed antibodies antibody Antigen antigens Beta Biomarker biosensors Blood body fluid circulation clustered regularly interspaced short palindromic repeats COVID-19 COVID-19 diagnosis COVID-19 virus diagnosis CRISPR Critical current Delta Detection sensitivity diagnostic early stage ELISA enzyme-linked immunosorbent enzyme-linked immunosorbent assay Gamma Infection isothermal amplification lateral flow immunoassay LFIA limit Loop-mediated isothermal amplification microarray analysis Microarray assays Mutation nucleic acid nucleic acids omicron outbreak overcome palindromic repeat physiological provide Rapid reduce reverse transcriptase-polymerase chain reaction reverse transcription RNA sequencing RT-LAMP RT-PCR Saliva SARS-CoV-2 Semen specificity Spread surface Urine variant variants virus viruses [DOI] 10.1016/j.trac.2022.116750 [Article Type] Review article
The DEAD box RNA helicase DDX42 is an intrinsic inhibitor of positive-strand RNA virusesArticle Published on 2022-11-072022-11-16 Journal: EMBO Reports [Category] SARS, 변종, [키워드] Antiviral approach cell line Complexes CRISPR DDX42 DDX42 DEAD-box RNA helicase Depletion DNA dominant-negative elements expression Helicase HIV-1 HIV-1 infection identify immunoprecipitation in vitro increase Infection inhibit inhibitor Interaction intrinsic intrinsic immunity LINE-1 mutant overexpression Perspective primary cells Replication restrict reverse transcription ribonucleoprotein RNA RNA virus RNA viruses RNA-seq analysis SARS-CoV-2 target cells viral infections viral inhibition. viruses [DOI] 10.15252/embr.202154061 PMC 바로가기
Use of Self-Collected Saliva Samples for the Detection of SARS-CoV-2Article Published on 2022-11-032022-11-16 Journal: Laboratory medicine [Category] SARS, 치료기술, [키워드] Accuracy Characteristics collected concordance rate cycle threshold Cycle threshold value detection drug Emergency use authorization EUA evaluated examined exhibiting FDA food individual nasopharyngeal Nasopharyngeal swab NP swab NP swabs over polymerase chain reference method reverse transcription RT-PCR Saliva saliva specimen Sample SARS-CoV-2 self-collection sensitivity Sensitivity. specimen Symptom Symptomatic patients tested [DOI] 10.1093/labmed/lmac051 PMC 바로가기
Reactive synovitis of the knee joint after COVID-19 vaccination: The first ultrastructural analysis of synovial fluidArticle Published on 2022-11-012022-11-15 Journal: International journal of rheumatic diseases [Category] SARS, 치료기술, [키워드] acute respiratory syndrome Administered adverse event affected Analysis Arthritis blood test clinical examination complex Consequences coronavirus coronavirus 2019 disease COVID-19 COVID-19 vaccination COVID-19 vaccine cytological-energy analysis detect Dexamethasone Diseases dose examined excluded Fever first dose follow-up period full recovery Infection intravenously joint Messenger RNA outcome Patient polymerase chain reactive Reactive arthritis recorded resulting reverse transcription SARS-CoV-2 Side-effects Spread Symptom synovial fluid synovial fluid. therapy Treatment Ultrastructural analysis Vaccine Vaccines virus with COVID-19 [DOI] 10.1111/1756-185X.14411 PMC 바로가기
Longitudinal serologic and viral testing post-SARS-CoV-2 infection and post-receipt of mRNA COVID-19 vaccine in a nursing home cohort-Georgia, October 2020‒April 2021Article Published on 2022-10-272022-11-15 Journal: PLoS ONE [Category] COVID19(2023년), SARS, 진단, 치료기술, [키워드] administration Antibody detection Antibody Response binding binding antibody blood collection Card Cohort conducted COVID-19 COVID-19 vaccination COVID-19 vaccine Culture detectable dose evaluate evaluated Final first dose geometric IgA antibodies IgA antibody IgG IgG titer IgM antibodies immune response Immunocompromised Infection longitudinal median age mounted mRNA nasal nine nursing home observation participant polymerase chain positive Post-infection Quantitative questionnaire reverse transcription robust RT-PCR SARS-CoV-2 SARS-CoV-2 antibody SARS-COV-2 infection SARS-CoV-2-specific immune response serology specimen subsequent vaccination viral culture [DOI] 10.1371/journal.pone.0275718 PMC 바로가기
Genomic Epidemiology and Serology Associated with a SARS-CoV-2 R.1 Variant Outbreak in New JerseyArticle Published on 2022-10-262022-11-15 Journal: mBio [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, 진단, 치료기술, [키워드] acute respiratory syndrome antibody antibody resistance attack rate B.1.351 B.1.526 Care caused characteristic Clinical use collected Community Complete coronavirus COVID-19 Critical E484K mutation Evidence Examining exhibit exhibited hcp Health highlight individual infected individuals Infection Inpatient Lineage loss of activity monoclonal antibody Mutation N-terminal domain neutralization capacity Neutralizing antibody response neutralizing capacity New nosocomial NTD outbreak P.1 Patient plasma positive professional professionals proportion pseudovirus psychiatric facility Receptor binding domain reported reverse transcription robust SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 variant sera Spike protein Symptom symptomatic COVID-19 disease tested the SARS-CoV-2 the United State treat vaccinated individual vaccinated individuals vaccination Vaccine variant variants of concern variants of concern. VOCs wild type [DOI] 10.1128/mbio.02141-22 PMC 바로가기
Delayed encephalopathy after COVID-19: A case series of six patientsArticle Published on 2022-10-212022-11-15 Journal: Medicine [Category] 진단, [키워드] absence accompanied acute respiratory syndrome Administered age autoantibody case sery cerebrospinal fluid characterized Computed tomography contribute coronavirus coronavirus disease COVID-19 CSF Delayed demonstrated disturbance of consciousness Duration dysfunction electroencephalogram elevated Encephalopathy evaluated female Hospitalization Immunoglobulin Immunotherapy interleukin-6 lacunar infarction magnetic resonance imaging males moderate MOST neurological Neurological deficit occur Pathogenesis Patient patients polymerase chain protein level pulse ranged remained Repeat resolved reverse transcription serum severity of COVID-19 Symptom Treatment with COVID-19 worsening [DOI] 10.1097/MD.0000000000031029 PMC 바로가기